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KMID : 0545119960060040295
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 4 p.295 ~ p.298
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The Effect of Glutamine on Production of Tissue-type Plasminogen Activator from Recombinant Human Melanoma Cells in Glutamine-limited Fed-batch Cultivation
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KIM, HYUN-GOO
KIM, TAE-HO/KIM, DAE-SEOK/PARK, KYUNG-YU/PARK, JIN-SEO/AHN, CHOL/LEE, JIN-HA
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Abstract
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Under glutamine-limited condition, 2×10^6 (viable cells/ml) of maximum cell density and 13.5 (㎍/ml) of tissue-type Plasminogen Activators (tPA) production were maintained by spike feeding fresh medium in fed-batch cultivation of human recombinant melanoma cells. It showed that tPA production was much seriously affected than cell growth according to initial glutamine concentrations. Above 3.4 (mmol/l) of glutamine concentration both cell growth and tPA production were not much affected by increasing initial glutamine concentration. Glutamine depleted situation was occurred at latter periods of batch and fed-batch cultivations below 5.4 (mmol/l) of initial glutamine concentration. It also showed that maximum glutamine consumption and ammonia evolution rates were closely related to initial glutamine concentrations. Maximum specific tPA production rate was estimated as 8.1×19^-6 (㎍/cells/h) at 3.4 (mmol/l) of glutamine concentration, which is higher than that from other batch and fed-batch processes.
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